To begin with, once you commence equipping
a molecular biology lab, you need the standard examination tubes, beakers,
desiccators, pipettes with fillers, and flasks, together with every one of the
essential clamps, stoppers, frames and supports.
The basic furniture will need to include
enough worktop space, personal computer station/s, chemical and organic storage
cupboards, perhaps a filtered, managed surroundings (heating & cooling, and
humidity control). In some cases access control, a gray room and clean room
conditions may even be necessary.
Since this branch of science deals with
organic materials from living cell tissues, it stands to reason that
temperatures are critical. Most laboratories use refrigerators and freezers,
and sometimes surface mounted refrigeration units. Some procedures may require
temperatures to be reduced to as low as -86A deg. C.
Centrifuges and microcentrifuges may ought
to stand on refrigerated and unrefrigerated tabletops for different purposes.
The heating devices may include a
microwave, Bunsen burners, and/or hot plates.
There are a number of calibrated and
measurement devices, apart from the glassware, that will be needed in a
laboratory, such as certain plastic ware, micro balances and scales, pH meters,
moisture meters and thermometers.
Microscopes or electron microscopes
appropriate to the research being done, along with camera port and link to
laptop. These are a part of every laboratory.
There is a large number of apparatus and
products generally considered essential to get a molecular biology laboratory.
Tissue processing for DNA identification is often an important part of
molecular biology and many processes require the gear for this, depending on
the extent of DNA study and genomics. Here is some of the general apparatus
likely to be needed:
* CO2 incubator for cell cultures.
* Liquid nitrogen tank.
* Spectrophotometer.
* Chromatograph.
* Vortex rotators and platform shakers.
* Autoclaves and sterilizers.
* Electrophoresis chamber and gel casting
and documentation chamber.
* UV Transilluminator.
* Gradient and standard thermocycler to
amplify segments of DNA.
Then we come to the question of safety and
protection of the atmosphere and of the people who work in the laboratory; and
most of the following will normally be essential:
Clothing such as:
* Aprons and lab coats.
* Coveralls.
* Footwear, such as shoe covers.
* Head covers, bouffant caps and face
covers, masks.
Gloves:
* Heavy weight latex gloves.
* Thin, disposable latex gloves.
* Nitrile gloves.
* Cotton gloves.
* Vinyl gloves.
For eye protection there is a great variety
of safety glasses, clear and tinted for lazer and UV work, as well as masks and
face shields with and without respirators for protection against splashing,
fumes and gasses and flying objects. It is important in a laboratory to install
an eyewash station for emergencies.
Last but not least is all of the equipment
needed for cleaning and sterilizing the glassware, apparatus and even the
clothing and household furniture used in the laboratory. The cleaning of these
items is not always straight forward, and some special procedures need to be
followed. You will need to have specially designed scrubbers and brushes as
well as suitable cleaning fluids and preparations, among other things.
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